Abstract
Two P450c11 genes, sharing 99% homology, were cloned from Cape baboon adrenal tissue using RT-PCR. The cDNAs showed 96% and 94.6% sequence identity to human P450c11 and aldosterone synthase, respectively. One of the gene sequences contained a termination codon in exon one. The cloned cDNAs were expressed in COS 1 cells and the metabolism of deoxycorticosterone to corticosterone investigated. The expressed enzyme exhibited 1 beta hydroxylase activity, but no aldosterone synthase activity.
MeSH terms
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Adrenal Glands / chemistry
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Animals
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COS Cells
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Cloning, Molecular
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Codon / genetics
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Corticosterone / metabolism
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Cytochrome P-450 Enzyme System / chemistry
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Cytochrome P-450 Enzyme System / genetics*
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DNA, Complementary / genetics
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Desoxycorticosterone / metabolism
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Exons / genetics
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Gene Expression
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Mixed Function Oxygenases / chemistry
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Mixed Function Oxygenases / genetics*
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Papio / genetics*
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Sequence Homology, Amino Acid
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Steroid 11-beta-Hydroxylase / metabolism
Substances
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Codon
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DNA, Complementary
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Desoxycorticosterone
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Cytochrome P-450 Enzyme System
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Mixed Function Oxygenases
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cytochrome P450 C11
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Steroid 11-beta-Hydroxylase
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Corticosterone