Enzyme assisted ensiling of alfalfa with enzymes by solid substrate fermentation

Bioresour Technol. 2001 Feb;76(3):207-12. doi: 10.1016/s0960-8524(00)00112-7.

Abstract

A crude enzyme preparation, obtained by solid substrate fermentation (SSF) with a Gliocladium spp. and added at the 5% level to wilted or non-wilted alfalfa, improved the fermentation characteristics and stability of alfalfa silages as effectively as commercial preparations, Novo-Nordisk Celluclast 1.5 L and Viscozyme 120 L, applied at the 0.025% level. The effective dose of the crude enzyme costs about one-fourth of the cost of the commercial enzymes.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Carbohydrate Metabolism
  • Dietary Fiber / metabolism
  • Fermentation
  • Gliocladium / enzymology*
  • Gliocladium / metabolism*
  • Lactic Acid / metabolism
  • Medicago sativa / metabolism*
  • Medicago sativa / microbiology
  • Silage* / economics
  • Silage* / microbiology

Substances

  • Dietary Fiber
  • Lactic Acid