Abstract
Here we show that the potential to regulate NFAT is a conserved property of different Nef alleles and that Nef residues involved in membrane targeting and SH3 binding are critical for this function. Cotransfection of an activated protein kinase C-theta (PKC-theta) with Nef implicated PKC-theta as a possible physiological cofactor of Nef in promoting NFAT-dependent gene expression and T-cell activation.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Alleles
-
Cell Membrane / metabolism
-
Conserved Sequence / genetics
-
DNA-Binding Proteins / genetics
-
DNA-Binding Proteins / metabolism*
-
Gene Products, nef / genetics
-
Gene Products, nef / metabolism*
-
Genes, nef*
-
HIV-1 / genetics
-
Humans
-
Isoenzymes / metabolism*
-
Jurkat Cells
-
NFATC Transcription Factors
-
Nuclear Proteins*
-
Protein Kinase C / metabolism*
-
Protein Kinase C-theta
-
Simian Immunodeficiency Virus / genetics
-
Transcription Factors / genetics
-
Transcription Factors / metabolism*
-
Transcriptional Activation*
-
Transfection
-
nef Gene Products, Human Immunodeficiency Virus
-
src Homology Domains / physiology
Substances
-
DNA-Binding Proteins
-
Gene Products, nef
-
Isoenzymes
-
NFATC Transcription Factors
-
Nuclear Proteins
-
Transcription Factors
-
nef Gene Products, Human Immunodeficiency Virus
-
PRKCQ protein, human
-
Protein Kinase C
-
Protein Kinase C-theta