The effect of zinc-crystallized glucagon-like peptide-1 on insulin secretion of macroencapsulated pancreatic islets

Tissue Eng. 2001 Feb;7(1):35-44. doi: 10.1089/107632701300003278.

Abstract

This research investigates the use of an insulinotropic factor, glucagon-like peptide-1 (GLP-1), to enhance insulin secretion from islets within a macrocapsule. A zinc-crystallized form of GLP-1 was added to the macrocapsule device to have a longer and more controlled release of the bioactive monomer GLP-1. The type of macrocapsule device used for this study consisted of a hollow fiber (MWCO 100,000 and 1 mm inner diameter) containing rat islets and GLP-1 crystals within a poly(N-isopropylacrylamide-co-acrylic acid) (2 mol% acrylic acid) matrix. When incubating the system in media with a high glucose concentration (300 mg/dL), insulin secretion was enhanced with a >85% increase after an induction period. When the same type of system was used in a dynamic perfusion experiment, similar results were obtained. GLP-1 crystals can be an effective form to be entrapped in a bioartificial pancreas to enhance insulin secretion function, especially at high glucose concentrations.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Survival
  • Crystallization
  • Glucagon / chemistry
  • Glucagon / metabolism*
  • Glucagon-Like Peptide 1
  • Insulin / metabolism*
  • Insulin Secretion
  • Islets of Langerhans / cytology
  • Islets of Langerhans / metabolism*
  • Male
  • Pancreas, Artificial*
  • Peptide Fragments / chemistry
  • Peptide Fragments / metabolism*
  • Perfusion
  • Polymers / chemistry
  • Protein Precursors / chemistry
  • Protein Precursors / metabolism*
  • Rats
  • Rats, Sprague-Dawley
  • Zinc / chemistry*

Substances

  • Insulin
  • Peptide Fragments
  • Polymers
  • Protein Precursors
  • Glucagon-Like Peptide 1
  • Glucagon
  • Zinc