Abstract
We studied the human immunodeficiency virus type 1 phenotypic and genotypic profiles of a dual drug-resistant isolate (isolate 14aPost-DR) selected for zidovudine (ZDV) and lamivudine (3TC) resistance and then cultured in the presence of 3TC and a protease inhibitor: indinavir (IDV), ritonavir, or KNI-272. The IDV-treated virus was highly resistant to 3TC, ZDV, and IDV and accumulated protease mutations at positions M46I and V82F. A change from alanine to valine was observed in 4 of 10 clones in the P2 position of the p7-p1 Gag-protease cleavage site, linked to position M46I in the dominant viral quasispecies. Previous 3TC resistance did not impair the development of additional mutations in the protease and Gag-protease cleavage regions.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Drug Resistance, Microbial
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Drug Resistance, Multiple
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Evolution, Molecular*
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Gene Products, gag / genetics*
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HIV Infections / virology
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HIV Protease / genetics*
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HIV Protease Inhibitors / pharmacology
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HIV-1 / drug effects*
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HIV-1 / enzymology
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HIV-1 / genetics
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HIV-1 / growth & development
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Humans
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Indinavir / pharmacology
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Lamivudine / pharmacology
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Microbial Sensitivity Tests
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Molecular Sequence Data
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Reverse Transcriptase Inhibitors / pharmacology*
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Zidovudine / pharmacology
Substances
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Gene Products, gag
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HIV Protease Inhibitors
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Reverse Transcriptase Inhibitors
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Lamivudine
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Zidovudine
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Indinavir
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HIV Protease
Associated data
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GENBANK/AF152964
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GENBANK/AF152965
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GENBANK/AF152966
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GENBANK/AF152967
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GENBANK/AF152968
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GENBANK/AF152969
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GENBANK/AF152970
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GENBANK/AF152971
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GENBANK/AF152972
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GENBANK/AF152973
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GENBANK/AF152974
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GENBANK/AF152975
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GENBANK/AF152976
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GENBANK/AF152977
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GENBANK/AF152978
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GENBANK/AF152979
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GENBANK/AF152980
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GENBANK/AF152981
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GENBANK/AF152982
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GENBANK/AF152983
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GENBANK/AF152984
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GENBANK/AF152985
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GENBANK/AF152986
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GENBANK/AF152987
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GENBANK/AF152988
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GENBANK/AF152989
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GENBANK/AF152990
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GENBANK/AF152991
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GENBANK/AF152992
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GENBANK/AF152993
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GENBANK/AF152994
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GENBANK/AF152995