Effects of beta-amyloid peptide on transient outward potassium current of acutely dissociated hippocampal neurons in CA1 sector in rats

Chin Med J (Engl). 1998 Jun;111(6):492-5.

Abstract

Objective: To explore the mechanism of beta-amyloid peptide (beta-AP) in Alzheimer's disease at ionic channel level.

Methods: Hippocampal CA1 neurons of 7-21 days' rats were acutely dissociated and the effects of beta-AP on transient outward potassium current were observed by a whole-cell recording patch clamp technique.

Results: beta-AP can significantly block transient potassium current in dose-dependent, time-dependent and partly voltage-dependent manners.

Conclusion: beta-AP may decrease the membrane conductance of K+ channels in hippocampal neurons, playing an important role in the pathophysiological mechanism of Alzheimer's disease.

MeSH terms

  • Amyloid beta-Peptides / pharmacology*
  • Animals
  • Depression, Chemical
  • Dose-Response Relationship, Drug
  • Hippocampus / metabolism*
  • Neurons / metabolism
  • Patch-Clamp Techniques
  • Peptide Fragments / pharmacology*
  • Potassium Channels / drug effects*
  • Potassium Channels / metabolism
  • Rats

Substances

  • Amyloid beta-Peptides
  • Peptide Fragments
  • Potassium Channels
  • amyloid beta-protein (25-35)