Increased expression of Fas (APO-1, CD95) on CD4+ and CD8+ T lymphocytes during total body irradiation

Acta Haematol. 2000;104(4):193-6. doi: 10.1159/000046514.

Abstract

Fas/APO-1 (CD95) is a cell surface molecule that can transduce apoptotic signals into cells. We examined the expression of Fas antigen on CD4+ and CD8+ T cells of patients who received total body irradiation (TBI) as a preparative regimen for allogeneic bone marrow transplantation. Numbers of peripheral blood lymphocytes were significantly reduced after TBI. Cytofluorometric analysis revealed a significantly higher expression of Fas on CD4+ and CD8+ T cells after TBI. Serum soluble Fas concentrations were significantly elevated after TBI. Changes in the Fas system were therefore accompanied by TBI-induced lymphocytopenia, suggesting that Fas plays a role in irradiation-induced apoptosis in vivo.

MeSH terms

  • Adult
  • Apoptosis / radiation effects
  • Bone Marrow Transplantation
  • CD4-Positive T-Lymphocytes / metabolism
  • CD4-Positive T-Lymphocytes / radiation effects
  • CD8-Positive T-Lymphocytes / metabolism
  • CD8-Positive T-Lymphocytes / radiation effects
  • Female
  • Flow Cytometry
  • Hematologic Neoplasms / complications
  • Hematologic Neoplasms / therapy
  • Humans
  • Lymphocyte Count
  • Lymphopenia / etiology
  • Male
  • T-Lymphocytes / metabolism*
  • Transplantation, Homologous
  • Up-Regulation
  • Whole-Body Irradiation*
  • fas Receptor / blood
  • fas Receptor / metabolism*
  • fas Receptor / radiation effects

Substances

  • fas Receptor