Overexpression of protein kinase C delta represses expression of proliferin in NIH3T3 cells that regulates cell proliferation

Mol Cell Biol Res Commun. 2000 Sep;4(3):181-7. doi: 10.1006/mcbr.2001.0276.

Abstract

Protein kinase C (PKC) delta is known to inhibit proliferation of many cell types. In this study we found that overexpression of PKCdelta reduced proliferation of NIH3T3 cells. To identify specific genes regulated by PKCdelta in regulation of cell proliferation, we used differential display-polymerase chain reaction in PKCdelta-overexpressing NIH3T3 cells and found that the expression of proliferin, a secreted protein known to stimulate cell proliferation, was significantly repressed. Transient transfection study indicated that the repression of proliferin expression was inversely proportional to the expression levels of PKCdelta. Addition of an anti-proliferin antibody to culture medium to neutralize the secreted proliferin decreased cell proliferation in a dose-dependent manner. Our results, therefore, suggest that overexpression of PKCdelta induces transcriptional repression of proliferin, thereby resulting in inhibition of cell proliferation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3 Cells
  • Animals
  • Blotting, Northern
  • Cell Division / drug effects
  • Cell Division / physiology*
  • Culture Media, Conditioned
  • Dose-Response Relationship, Drug
  • Gene Expression Profiling
  • Gene Expression Regulation*
  • Glycoproteins / genetics
  • Glycoproteins / immunology
  • Glycoproteins / metabolism*
  • Growth Substances / genetics
  • Growth Substances / metabolism*
  • Immunoblotting
  • Intercellular Signaling Peptides and Proteins
  • Isoenzymes / genetics
  • Isoenzymes / metabolism*
  • Mice
  • Microscopy, Fluorescence
  • Placenta / cytology
  • Prolactin
  • Protein Kinase C / genetics
  • Protein Kinase C / metabolism*
  • Protein Kinase C-alpha
  • Protein Kinase C-delta
  • Recombinant Proteins / metabolism
  • Tetradecanoylphorbol Acetate / pharmacology
  • Transfection

Substances

  • Culture Media, Conditioned
  • Glycoproteins
  • Growth Substances
  • Intercellular Signaling Peptides and Proteins
  • Isoenzymes
  • Prl2c2 protein, mouse
  • Recombinant Proteins
  • Prolactin
  • Prkcd protein, mouse
  • Prkca protein, mouse
  • Protein Kinase C
  • Protein Kinase C-alpha
  • Protein Kinase C-delta
  • Tetradecanoylphorbol Acetate