A new human immunodeficiency virus type 1 circulating recombinant form from Tanzania

AIDS Res Hum Retroviruses. 2001 Mar 20;17(5):423-31. doi: 10.1089/088922201750102508.

Abstract

It is becoming increasingly important to identify and to study human immunodeficiency virus type 1 (HIV-1) circulating recombinant forms (CRFs) with evidence of epidemic spread, since mosaic strains arise frequently, especially in populations where multiple subtypes cocirculate. We describe the almost complete nucleotide sequence of 3 subtype C and D recombinant viruses, selected from a pool of 13 D(gag)-D/C/D(env) perinatally infected infants from Dar es Salaam, Tanzania. All three genomes had cross-over points with approximately the same genomic localization. The subtype C-like sequences were located within pol, vif, vpr, vpu, the first exons of rev and tat, V3, and the U3-R regions of the LTR. Phylogenetic analyses of the full-length genomic sequences from these viruses showed the formation of a distinct subcluster on the HIV-1 subtype D branch. The pattern of recombination of genomes belonging to this new CRF, named CRF10_CD, might have resulted from independent recombination events occurring at high frequency or from a single source that originated earlier in this population. Future surveys will be needed to determine the potential of this CRF for epidemic spread.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Consensus Sequence
  • Genome, Viral
  • HIV Infections / congenital
  • HIV Infections / epidemiology
  • HIV Infections / virology*
  • HIV-1 / chemistry
  • HIV-1 / genetics*
  • HIV-1 / isolation & purification
  • Humans
  • Infant
  • Phylogeny
  • Recombination, Genetic / genetics*
  • Tanzania