Evaluation of PCR methods for rapid identification and differentiation of Streptococcus uberis and Streptococcus parauberis

A A Hassan; I U Khan; A Abdulmawjood; C Lämmler

doi:10.1128/JCM.39.4.1618-1621.2001

Evaluation of PCR methods for rapid identification and differentiation of Streptococcus uberis and Streptococcus parauberis

J Clin Microbiol. 2001 Apr;39(4):1618-21. doi: 10.1128/JCM.39.4.1618-1621.2001.

Authors

A A Hassan¹, I U Khan, A Abdulmawjood, C Lämmler

Affiliation

¹ Institut für Tierärztliche Nahrungsmittelkunde, Bakteriologie und Hygiene der Milch, der Justus-Liebig-Universität Giessen, Giessen, Germany.

Abstract

Streptococcus uberis and Streptococcus parauberis reference strains and isolates obtained from routine diagnostics were investigated by PCR with oligonucleotide primers designed according to species-specific parts of the 16S rRNA gene, the 23S rRNA gene, and the 16S-23S rRNA intergenic spacer region of both species. All three primer pairs allowed an identification of 67 isolates as S. uberis and 4 isolates as S. parauberis.

Publication types

Evaluation Study

MeSH terms

Animals
Base Sequence
Cattle
Cattle Diseases / microbiology
DNA, Ribosomal Spacer / genetics*
Genes, rRNA*
Mastitis, Bovine / microbiology*
Molecular Sequence Data
Polymerase Chain Reaction / methods*
RNA, Ribosomal, 16S / genetics
RNA, Ribosomal, 23S / genetics
Sequence Analysis, DNA
Streptococcal Infections / microbiology
Streptococcal Infections / veterinary*
Streptococcus / classification*
Streptococcus / genetics
Time Factors

Substances

DNA, Ribosomal Spacer
RNA, Ribosomal, 16S
RNA, Ribosomal, 23S

Associated data

GENBANK/AF255656
GENBANK/AF255657