Identification of a human brain-specific gene, calneuron 1, a new member of the calmodulin superfamily

Mol Genet Metab. 2001 Apr;72(4):343-50. doi: 10.1006/mgme.2001.3160.

Abstract

The calmodulin superfamily includes the calmodulins, calcium-binding proteins, and related genes. Herein, we describe the cloning and characterization of human calneuron 1 (CALN1). CALN1 encodes a novel neuron-specific protein that maps to chromosome 7q11. CALN1 spans a large genomic region (>360 kb). Sequence comparison shows significant similarity with the calmodulin superfamily of genes, especially in the two conserved EF-hand motifs. The mouse orthologous gene (Caln1) shows little prenatal expression, with highest expression at Postnatal Day 21. In situ hybridization to adult mouse brain shows high expression in the cerebellum, hippocampus, and cortex. The high expression of this gene exclusively in brain, the developmental changes in expression levels, the high homology with calmodulin which indicates a potential role in signal transduction, and the cellular localization of the mRNA suggest that CALN1 has a significant role in the physiology of neurons and is potentially important in memory and learning.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Blotting, Northern
  • Brain / metabolism*
  • Brain Chemistry / genetics*
  • Calmodulin / genetics*
  • Calmodulin / metabolism
  • Chromosome Mapping
  • Chromosomes, Human, Pair 7 / genetics
  • Cloning, Molecular
  • DNA Primers / chemistry
  • DNA, Complementary / analysis
  • DNA, Complementary / isolation & purification
  • Gene Expression
  • Humans
  • In Situ Hybridization, Fluorescence
  • Mice
  • Molecular Sequence Data
  • Nerve Tissue Proteins / genetics*
  • Nerve Tissue Proteins / metabolism
  • Phosphorylation
  • Polymerase Chain Reaction
  • Protein Binding
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid

Substances

  • CALN1 protein, human
  • Calmodulin
  • DNA Primers
  • DNA, Complementary
  • Nerve Tissue Proteins

Associated data

  • GENBANK/AF282250
  • GENBANK/AF282251