Abstract
Transport of BPV-1 virus from the cell membrane to the nucleus was studied in vitro in CV-1 cells. At reduced temperature (4 degrees C), BPV-1 binding to CV-1 cells was unaffected but there was no transport of virions across the cytosol. Electron microscopy showed BPV-1 virions in association with microtubules in the cytoplasm, a finding confirmed by co-immunoprecipitation of L1 protein and tubulin. Internalization of virus was unimpaired in cells treated with the microtubule-depolymerizing drug nocodazole but virions were retained in cytoplasmic vesicles and not transported to the nucleus. We conclude that a microtubule transport mechanism in CV-1 cells moves intact BPV-1 virions from the cell surface to the nuclear membrane.
Copyright 2001 Academic Press.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Active Transport, Cell Nucleus / drug effects
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Animals
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Blotting, Western
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Bovine papillomavirus 1 / drug effects
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Bovine papillomavirus 1 / metabolism*
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Bovine papillomavirus 1 / physiology
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Bovine papillomavirus 1 / ultrastructure
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Capsid / metabolism
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Cattle
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Cell Line
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Cell Membrane / drug effects
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Cell Membrane / metabolism
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Cell Membrane / virology
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Cell Nucleus / drug effects
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Cell Nucleus / metabolism
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Cell Nucleus / virology
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Cytoplasmic Vesicles / drug effects
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Cytoplasmic Vesicles / metabolism
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Cytoplasmic Vesicles / virology
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Fluorescent Antibody Technique, Indirect
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Microscopy, Electron
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Microtubules / chemistry
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Microtubules / drug effects
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Microtubules / metabolism*
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Microtubules / ultrastructure
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Nocodazole / pharmacology
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Paclitaxel / pharmacology
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Protein Binding
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Temperature
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Tubulin / metabolism
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Virion / drug effects
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Virion / metabolism
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Virion / physiology
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Virion / ultrastructure
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Warts / virology
Substances
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Tubulin
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Paclitaxel
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Nocodazole