CD62L is required on effector cells for local interactions in the CNS to cause myelin damage in experimental allergic encephalomyelitis

Immunity. 2001 Mar;14(3):291-302. doi: 10.1016/s1074-7613(01)00110-8.

Abstract

Adhesion molecules are believed to facilitate infiltration of leukocytes into the CNS of mice with experimental allergic encephalomyelitis (EAE). The role of the adhesion molecule CD62L (L-selectin) in the immunopathology of EAE is not known. To study this, we crossed CD62L-deficient mice with myelin basic protein-specific TCR (MBP-TCR) transgenic mice. CD62L-deficient MBP-TCR transgenic mice failed to develop antigen-induced EAE, and, despite the presence of leukocyte infiltration, damage to myelin in the CNS was not seen. EAE could, however, be induced in CD62L-deficient mice upon adoptive transfer of wild-type macrophages. Our results suggest that CD62L is not required for activation of autoimmune CD4 T cells but is important for the final destructive function of effector cells in the CNS and support a novel mechanism whereby CD62L expressed on effector cells is important in mediating myelin damage.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adoptive Transfer
  • Animals
  • Antigen-Presenting Cells / immunology
  • Antigen-Presenting Cells / metabolism
  • Antigen-Presenting Cells / transplantation
  • Autoimmunity / genetics
  • Autoimmunity / immunology
  • B-Lymphocytes / immunology
  • B-Lymphocytes / metabolism
  • CD4-Positive T-Lymphocytes / immunology
  • CD4-Positive T-Lymphocytes / metabolism
  • CD4-Positive T-Lymphocytes / transplantation
  • Cell Adhesion
  • Central Nervous System / metabolism*
  • Central Nervous System / pathology
  • Chemotaxis, Leukocyte
  • Encephalomyelitis, Autoimmune, Experimental / metabolism*
  • Encephalomyelitis, Autoimmune, Experimental / pathology*
  • Gene Deletion
  • Immunohistochemistry
  • L-Selectin / genetics
  • L-Selectin / metabolism*
  • Lymphocyte Activation
  • Macrophages / immunology
  • Macrophages / metabolism
  • Macrophages / transplantation
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mice, Transgenic
  • Myelin Basic Protein / genetics
  • Myelin Basic Protein / immunology
  • Myelin Basic Protein / metabolism
  • Myelin Sheath / metabolism
  • Myelin Sheath / pathology*
  • Receptors, Antigen, T-Cell / genetics
  • Receptors, Antigen, T-Cell / immunology
  • Receptors, Antigen, T-Cell / metabolism
  • Recombinant Fusion Proteins / metabolism

Substances

  • Myelin Basic Protein
  • Receptors, Antigen, T-Cell
  • Recombinant Fusion Proteins
  • L-Selectin