Abstract
The distribution of ribosomes was investigated in mature human mast cells with a chelation-based staining protocol known to bleach DNA-rich structures, leaving RNA-rich structures unbleached. With this method, electron-dense ribosomes were adjacent to, attached to, and within secretory granules, which were also bleached with the chelation method that we used. The finding of these ribosome-secretory granule relationships suggests that secretory granules in mature human mast cells may participate in RNA metabolism.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Chelating Agents / analysis*
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Citrates / analysis
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Coloring Agents / analysis
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Cytoplasmic Granules / ultrastructure*
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Edetic Acid / analysis*
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Humans
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Lead / analysis
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Lung / cytology
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Mast Cells / ultrastructure*
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Microscopy, Electron
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Organometallic Compounds / analysis
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Ribonucleoproteins / analysis
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Ribosomes / ultrastructure*
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Staining and Labeling / methods*
Substances
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Chelating Agents
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Citrates
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Coloring Agents
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Organometallic Compounds
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Ribonucleoproteins
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uranyl acetate
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Lead
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Edetic Acid