Abstract
Two full length cDNA sequences encoding the kappa light chain of the Australian marsupial, Trichosurus vulpecula, the brushtail possum, were isolated from a mesenteric lymph node cDNA library. The constant regions (Ckappa) of the two light chains were identical, but the variable (Vkappa) and joining (Jkappa) regions were different. At the amino acid level, possum Ckappa was most similar to Ckappa of an American marsupial, Monodelphis domestica (75%), with similarity to eutherian Ckappa ranging from 47 to 63%. The availability of molecular data will enable the development of immunological reagents for studying immune responses and disease in marsupials, thereby aiding conservation strategies and veterinary medicine.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Anura
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Australia
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DNA, Complementary / chemistry
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Fishes
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Haplorhini
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Humans
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Immunoglobulin Constant Regions / chemistry
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Immunoglobulin Constant Regions / genetics
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Immunoglobulin Light Chains / chemistry*
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Immunoglobulin Light Chains / genetics
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Immunoglobulin Variable Region / chemistry
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Immunoglobulin Variable Region / genetics
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Immunoglobulin kappa-Chains / chemistry*
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Immunoglobulin kappa-Chains / genetics
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Marsupialia / immunology*
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Mink
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Molecular Sequence Data
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Opossums
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Rabbits
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Sequence Alignment
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Swine
Substances
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DNA, Complementary
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Immunoglobulin Constant Regions
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Immunoglobulin Light Chains
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Immunoglobulin Variable Region
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Immunoglobulin kappa-Chains
Associated data
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GENBANK/AF321284
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GENBANK/AF321285