Design of novel zinc finger proteins: towards artificial control of specific gene expression

Eur J Pharm Sci. 2001 Apr;13(1):91-7. doi: 10.1016/s0928-0987(00)00212-8.

Abstract

In this review, we summarize design strategies for generating proteins with desired sequences such as long contiguous base pairs and diverse sequence specificities based on the nature of Cys(2)-His(2) zinc finger proteins. Recent progress towards artificial DNA binding proteins has been achieved by structure-based design processes and by selection strategies. Indeed, a multi-zinc finger protein with an 18 (or 27)-base pair address, and new zinc finger proteins for diverse DNA target sites (TATA-box and p53 binding site) have been created successfully. Such novel zinc finger proteins will probably be useful tools in molecular biology and potentially in human medicine.

MeSH terms

  • Animals
  • DNA / metabolism
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / metabolism*
  • Early Growth Response Protein 1
  • Gene Expression / physiology*
  • Genes, p53 / physiology
  • Humans
  • Immediate-Early Proteins*
  • TATA Box / physiology
  • Transcription Factors / chemistry
  • Transcription Factors / metabolism*
  • Transcriptional Activation / drug effects
  • Transcriptional Activation / physiology*

Substances

  • DNA-Binding Proteins
  • EGR1 protein, human
  • Early Growth Response Protein 1
  • Immediate-Early Proteins
  • Transcription Factors
  • DNA