Abstract
There are two distinct nickel resistance loci on plasmid pTOM9 from Achromobacter xylosoxidans 31A, ncc and nre. Expression of the nreB gene was specifically induced by nickel and conferred nickel resistance on both A. xylosoxidans 31A and Escherichia coli. E. coli cells expressing nreB showed reduced accumulation of Ni(2+), suggesting that NreB mediated nickel efflux. The histidine-rich C-terminal region of NreB was not essential but contributed to maximal Ni(2+) resistance.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Alcaligenes / drug effects
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Alcaligenes / genetics*
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Alcaligenes / metabolism
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Blotting, Northern
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Carrier Proteins / biosynthesis
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Carrier Proteins / genetics*
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Drug Resistance, Microbial
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Escherichia coli / drug effects
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Escherichia coli / genetics
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Genes, Bacterial*
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Microbial Sensitivity Tests
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Mutagenesis, Insertional
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Nickel / pharmacology
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Plasmids
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RNA, Bacterial / analysis
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RNA, Messenger / analysis
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Recombinant Proteins / biosynthesis
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Transcription, Genetic
Substances
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Carrier Proteins
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RNA, Bacterial
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RNA, Messenger
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Recombinant Proteins
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Nickel