Ontogeny, distribution and function of CD38-expressing B lymphocytes in mice

Eur J Immunol. 2001 Apr;31(4):1261-7. doi: 10.1002/1521-4141(200104)31:4<1261::AID-IMMU1261gt;3.0.CO;2-H.

Abstract

Analysis of expression of CD38, CD45R (B220), IgM and IgD on splenic B lymphocytes from mice of different ages demonstrated CD38 on both immature (B220(+), BCR(-)) and mature (B220(+), BCR(+)) B lymphocytes. Similarly, CD38 is expressed as early as B220 on the surface of progenitor B cells in the bone marrow. In spite of expressing of CD38 and IgM, neonatal B cells, in contrast to the adult, failed to proliferate to either anti-CD38 or anti-IgM cross-linking when IL-4 was present. They did, however, respond to LPS and anti-CD40, and by 2 weeks of age they began to respond to anti-CD38 and anti-IgM, reaching adult B cell levels by 4 weeks. Although the distribution of CD38 on adult B cells from most different lymphoid compartments was broadly similar, significantly higher levels of CD38 were expressed on peritoneal B lymphocytes. A detailed analysis, using IgM / IgD ratio and staining with anti-CD5 confirmed that B1 lymphocytes were expressing a high level of CD38. Interestingly, both immature B cells and peritoneal B1 lymphocytes were unresponsive to anti-CD38. However, they were activated by LPS or anti-CD40.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ADP-ribosyl Cyclase
  • ADP-ribosyl Cyclase 1
  • Aging / immunology
  • Animals
  • Antibodies
  • Antigens, CD*
  • Antigens, Differentiation / analysis
  • Antigens, Differentiation / immunology
  • Antigens, Differentiation / metabolism*
  • B-Lymphocytes / cytology
  • B-Lymphocytes / immunology*
  • B-Lymphocytes / metabolism*
  • Bone Marrow Cells / immunology
  • Bone Marrow Cells / metabolism
  • CD40 Antigens / immunology
  • Cell Differentiation
  • Cell Division
  • Female
  • Flow Cytometry
  • Immunoglobulin D / analysis
  • Immunoglobulin D / immunology
  • Immunoglobulin M / analysis
  • Immunoglobulin M / immunology
  • Lipopolysaccharides / immunology
  • Lymphocyte Activation
  • Membrane Glycoproteins
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C3H
  • Mice, Inbred C57BL
  • NAD+ Nucleosidase / analysis
  • NAD+ Nucleosidase / immunology
  • NAD+ Nucleosidase / metabolism*
  • Peritoneal Cavity / cytology
  • Peyer's Patches / immunology
  • Receptor Aggregation
  • Spleen / immunology
  • Stem Cells / cytology
  • Stem Cells / immunology
  • Stem Cells / metabolism

Substances

  • Antibodies
  • Antigens, CD
  • Antigens, Differentiation
  • CD40 Antigens
  • Immunoglobulin D
  • Immunoglobulin M
  • Lipopolysaccharides
  • Membrane Glycoproteins
  • ADP-ribosyl Cyclase
  • Cd38 protein, mouse
  • NAD+ Nucleosidase
  • ADP-ribosyl Cyclase 1