Radioimmunoassay for somatostatin receptor type 2

Endocr Regul. 2001 Mar;35(1):31-4.

Abstract

Objective: To develop radioimmunoassay for somatostatin receptor type 2 (SSTR2) and search for its presence in certain rat tissues.

Methods: Anti-SSTR2 antiserum has been raised in New Zealand white rabbits immunized with a conjugate of synthetic SSTR2 with bovine serum albumin. Radioiodination of SSTR2 was performed by chloramin T method followed by purification of radioiodinated material on Sephadex G-25 column.

Results: The obtained antibody did not crossreact with SSTR1, SSTR3, SSTR4, SSTR5, hypothalamic hormones, pituitary hormones, neuropeptides or gut hormones. The assay was performed with a double antibody system. SSTR2 was extracted from the tissues with acid acetone. The dilution curve of acid acetone-extracts of rat hypothalamus in the radioimmunoassay system was parallel to the standard curve. The recovery of tissue SSTR2 was about 89 %, and the intra-assay and inter-assay variations were 4.9 % and 7.8 %, respectively. SSTR2 was found in the hypothalamus, cerebrum, cerebellum, pituitary, stomach and testis.

Conclusions: These data suggest that this assay system is suitable for the estimation of SSTR2 in the tissues.

MeSH terms

  • Acetone
  • Animals
  • Antibody Specificity
  • Brain Chemistry
  • Cerebellum / chemistry
  • Hypothalamus / chemistry
  • Immune Sera
  • Iodine Radioisotopes
  • Isotope Labeling
  • Male
  • Pituitary Gland / chemistry
  • Quality Control
  • Rabbits
  • Rats
  • Rats, Wistar
  • Receptors, Somatostatin / analysis*
  • Stomach / chemistry
  • Telencephalon / chemistry
  • Testis / chemistry

Substances

  • Immune Sera
  • Iodine Radioisotopes
  • Receptors, Somatostatin
  • Acetone
  • somatostatin receptor 2