Abstract
The gp120 V3-encoding region of human immunodeficiency virus type 1 (HIV-1) RNA derived from the saliva and blood plasma of 11 individuals was characterized by heteroduplex tracking assay and sequence analyses. R5-like viral variants were identified in both fluids of all subjects. X4-like variants were detected in the plasma and/or saliva of three subjects, indicating that X4-like variants are not excluded from the saliva compartment. Viral subpopulations were similar in both fluids of most subjects, suggesting that HIV-1 in oral fluids and blood may stem from a common source. These findings raise the possibility of using saliva as a noninvasive fluid for evaluating and monitoring viral evolution in infected persons.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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DNA, Viral
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Genotype
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HIV Envelope Protein gp120 / genetics*
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HIV Infections / virology*
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HIV-1 / classification
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HIV-1 / genetics*
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Heteroduplex Analysis
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Humans
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Molecular Sequence Data
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Peptide Fragments / genetics*
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Plasma / virology
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Saliva / virology
Substances
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DNA, Viral
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HIV Envelope Protein gp120
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HIV envelope protein gp120 (305-321)
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Peptide Fragments
Associated data
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GENBANK/AF362846
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GENBANK/AF362847
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GENBANK/AF362848
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GENBANK/AF362849
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GENBANK/AF362850
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GENBANK/AF362851
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GENBANK/AF362852
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GENBANK/AF362853
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GENBANK/AF362854
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GENBANK/AF362855
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GENBANK/AF362856
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GENBANK/AF362857
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GENBANK/AF362858
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GENBANK/AF362859
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GENBANK/AF362860
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GENBANK/AF362861
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GENBANK/AF362862
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GENBANK/AF362863
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GENBANK/AF362864
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GENBANK/AF362865
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GENBANK/AF362866
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GENBANK/AF362867
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GENBANK/AF362868
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GENBANK/AF362869
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GENBANK/AF362870
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GENBANK/AF362871
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GENBANK/AF362872
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GENBANK/AF362873
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GENBANK/AF362874
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GENBANK/AF362875
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GENBANK/AF362876
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GENBANK/AF362877
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GENBANK/AF362878
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GENBANK/AF362879
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GENBANK/AF362880
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GENBANK/AF362881
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GENBANK/AF362882
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GENBANK/AF362883
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GENBANK/AF362884
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GENBANK/AF362885