Effect of the interaction between a low tyramine-producing Lactobacillus and proteolytic staphylococci on biogenic amine production during ripening and storage of dry sausages

Int J Food Microbiol. 2001 Apr 11;65(1-2):113-23. doi: 10.1016/s0168-1605(00)00525-0.

Abstract

The interaction between tyrosine-decarboxylase and proteolytic activities of a Lactobacillus curvatus and Staphylococcus xylosus, respectively, on biogenic amine production during the ripening and the storage of dry fermented sausages was investigated. Water content, pH, proteolysis parameters, microbial counts, and biogenic amine contents were monitored in spontaneously and starter fermented sausages. The use of proteolytic staphylococci as starter resulted in a higher content of non-protein nitrogen and total free amino acids. Tyramine was the main amine produced in all batches. However, tyrosine-decarboxylase activity of the L. curvatus starter strain was weak and yielded lower amounts of tyramine than those produced by the wild mioroflora in the control batch. Association between tyramine production and proteolysis could only be established in a defectively dried batch. Putrescine and cadaverine accumulation was efficiently reduced in the starter-mediated fermentation, in agreement with the lower development of enterobacteria. Phenylethylamine and tryptamine were only detected in the spontaneously fermented sausages, while histamine, spermine and spermidine did not vary during the ripening. Biogenic amine levels and related parameters showed significant changes during the storage of dry sausages depending on the temperature and the batch. As a general rule, changes in the pH, proteolysis, microbial counts, and biogenic amine contents were stronger at 19 degrees C than at 4 degrees C. The results suggest that refrigeration would be advisable for preventing further accumulation of biogenic amines during the storage of dry fermented sausages.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biogenic Amines / biosynthesis*
  • Colony Count, Microbial
  • Fermentation
  • Food Handling
  • Food Preservation*
  • Hydrogen-Ion Concentration
  • Lactobacillus / metabolism*
  • Meat Products / microbiology*
  • Staphylococcus / metabolism*
  • Temperature
  • Tyramine / biosynthesis
  • Water

Substances

  • Biogenic Amines
  • Water
  • Tyramine