Does blood of healthy subjects contain bacterial ribosomal DNA?

S Nikkari; I J McLaughlin; W Bi; D E Dodge; D A Relman

doi:10.1128/JCM.39.5.1956-1959.2001

Does blood of healthy subjects contain bacterial ribosomal DNA?

J Clin Microbiol. 2001 May;39(5):1956-9. doi: 10.1128/JCM.39.5.1956-1959.2001.

Authors

S Nikkari¹, I J McLaughlin, W Bi, D E Dodge, D A Relman

Affiliation

¹ Department of Microbiology and Immunology and Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA. [email protected]

Abstract

Real-time PCR methods with primers and a probe targeting conserved regions of the bacterial 16S ribosomal DNA (rDNA) revealed a larger amount of rDNA in blood specimens from healthy individuals than in matched reagent controls. However, the origins and identities of these blood-associated bacterial rDNA sequences remain obscure.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Bacteria / genetics
DNA Primers / genetics
DNA, Bacterial / blood*
DNA, Ribosomal / blood*
Humans
Polymerase Chain Reaction / methods*
RNA, Ribosomal, 16S / genetics*

Substances

DNA Primers
DNA, Bacterial
DNA, Ribosomal
RNA, Ribosomal, 16S