Effects of high-fat, low-cholesterol diets on hepatic lipid peroxidation and antioxidants in apolipoprotein E-deficient mice

Mol Cell Biochem. 2001 Feb;218(1-2):165-9. doi: 10.1023/a:1007296919243.

Abstract

The present study describes the effects of several high-fat low-cholesterol antiatherogenic diets on the hepatic lipid peroxidation and hepatic antioxidant systems in apolipoprotein E-deficient mice. Eighty mice were distributed into five groups and fed with regular mouse chow or chow supplemented with coconut, palm, olive and sunflower seed oils. After ten weeks, they were sacrificed and the livers were removed so that lipid peroxidation and alpha-tocopherol concentrations, and superoxide dismutase, glutathione peroxidase and glutathione reductase activities could be measured. The size of the atherosclerotic lesions in the aortas was also measured. Results showed that the diets supplemented with olive oil, palm oil or sunflower seed oil significantly decreased the size of the lesion. However, there was an association between those mice that were on diets supplemented with palm or coconut oils and a significant increase in hepatic lipid peroxidation. This association was not found in animals fed with olive or sunflower seed oils, the diets with the highest content of vitamin E. The dietary content of vitamin E was significantly correlated (r = 0.98; p < 0.05) with the hepatic concentration of this compound. Our study suggests that the high content of vitamin E in olive oil or sunflower seed oil may protect from the undesirable hepatotoxic effects of high-fat diets in apo E-deficient mice and that this should be taken into account when these diets are used to prevent atherosclerosis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants
  • Aorta / pathology
  • Apolipoproteins E / blood
  • Apolipoproteins E / deficiency*
  • Apolipoproteins E / genetics
  • Arteriosclerosis / drug therapy*
  • Arteriosclerosis / genetics
  • Cholesterol, Dietary / blood*
  • Cholesterol, Dietary / pharmacology*
  • Dietary Fats*
  • Female
  • Gene Expression / physiology
  • Glutathione Peroxidase / metabolism
  • Glutathione Reductase / metabolism
  • Inbreeding
  • Lipid Peroxidation / drug effects*
  • Lipids / blood
  • Liver / enzymology
  • Liver / metabolism*
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred Strains
  • Mice, Knockout
  • Oxidation-Reduction
  • Plant Oils / pharmacology
  • RNA, Messenger / analysis
  • Superoxide Dismutase / metabolism
  • Thiobarbituric Acid Reactive Substances / analysis
  • Thiobarbituric Acid Reactive Substances / metabolism
  • Vitamin E / metabolism
  • Vitamin E / therapeutic use

Substances

  • Antioxidants
  • Apolipoproteins E
  • Cholesterol, Dietary
  • Dietary Fats
  • Lipids
  • Plant Oils
  • RNA, Messenger
  • Thiobarbituric Acid Reactive Substances
  • Vitamin E
  • Glutathione Peroxidase
  • Superoxide Dismutase
  • Glutathione Reductase