A new multiplex PCR strategy for the simultaneous determination of four genetic polymorphisms affecting HIV-1 disease progression

J Immunol Methods. 2001 Jun 1;252(1-2):147-51. doi: 10.1016/s0022-1759(01)00349-0.

Abstract

The CCR5 Delta32, CCR2 64I, SDF1 3'A, and CCR5 promoter 59029 polymorphisms have been suggested to influence HIV-1 disease progression. Furthermore, the CCR5 Delta32 and the CCR2 64I polymorphisms have been associated with various other diseases. The purpose of the present study was to develop a multiplex assay for the simultaneous determination of these four polymorphisms. Results obtained with the multiplex assay were compared to results obtained by conventional RFLP-PCR and no differences were observed. The multiplex assay offers a quick tool for the determination of the CCR5 Delta32, CCR2 64I, SDF1 3'A, and CCR5 promoter 59029 A/G polymorphisms.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chemokine CXCL12
  • Chemokines, CXC / genetics*
  • Disease Progression
  • HIV Infections / genetics*
  • HIV Infections / physiopathology
  • HIV-1 / physiology*
  • Humans
  • Polymerase Chain Reaction / methods*
  • Polymorphism, Genetic*
  • Receptors, CCR2
  • Receptors, CCR5 / genetics*
  • Receptors, Chemokine / genetics*

Substances

  • CCR2 protein, human
  • CXCL12 protein, human
  • Chemokine CXCL12
  • Chemokines, CXC
  • Receptors, CCR2
  • Receptors, CCR5
  • Receptors, Chemokine