Human myoblast fusion requires expression of functional inward rectifier Kir2.1 channels

J Cell Biol. 2001 May 14;153(4):677-86. doi: 10.1083/jcb.153.4.677.

Abstract

Myoblast fusion is essential to skeletal muscle development and repair. We have demonstrated previously that human myoblasts hyperpolarize, before fusion, through the sequential expression of two K+ channels: an ether-à-go-go and an inward rectifier. This hyperpolarization is a prerequisite for fusion, as it sets the resting membrane potential in a range at which Ca2+ can enter myoblasts and thereby trigger fusion via a window current through alpha1H T channels.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antisense Elements (Genetics)
  • Child
  • Child, Preschool
  • Humans
  • In Vitro Techniques
  • Infant
  • Membrane Fusion / physiology
  • Membrane Potentials / physiology
  • Muscle Fibers, Skeletal / cytology*
  • Muscle, Skeletal / cytology*
  • Muscle, Skeletal / physiology
  • Patch-Clamp Techniques
  • Potassium / metabolism
  • Potassium Channels / genetics*
  • Potassium Channels / metabolism*
  • Potassium Channels, Inwardly Rectifying*
  • Ribonucleases

Substances

  • Antisense Elements (Genetics)
  • Potassium Channels
  • Potassium Channels, Inwardly Rectifying
  • Ribonucleases
  • Potassium