Intracerebroventricular CART peptide reduces rat ingestive behavior and alters licking microstructure

Am J Physiol Regul Integr Comp Physiol. 2001 Jun;280(6):R1613-9. doi: 10.1152/ajpregu.2001.280.6.R1613.

Abstract

Intracerebroventricular administration of cocaine- and amphetamine-regulated transcript (CART) peptides reduces food intake and increases c-Fos in brain areas involved in the control of feeding. To discern behavioral mechanisms through which CART alters the microstructure of feeding, we injected CART-(55--102) (0.1, 0.5, 1, 2 microg, and saline controls) into the lateral ventricle of male Sprague-Dawley rats 5 min before dark onset and, using lickometers, monitored the ingestion of an Ensure liquid diet for the first 6 h of dark. At a threshold dose of 1 microg, CART dose dependently 1) decreased intake of Ensure in licks; 2) decreased meal size, but did not alter meal duration or number; 3) reduced initial lick rate of meals; and 4) significantly reduced burst number, licks/burst, and licks/cluster. CART dose dependently increased interlick interval (0.5 microg threshold, 192 +/- 4 vs. 183 +/- 3 ms, control; 1 microg: 201 +/- 1 ms; 2 microg: 214 +/- 6 ms). These data suggest that altered oral motor function, and possibly palatability perception, may be fundamental to the anorexigenic action of CART.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Behavior, Animal / drug effects*
  • Cocaine- and Amphetamine-Regulated Transcript Protein
  • Dose-Response Relationship, Drug
  • Feeding Behavior / drug effects*
  • Injections, Intraventricular
  • Male
  • Nerve Tissue Proteins / administration & dosage*
  • Nerve Tissue Proteins / pharmacology
  • Rats
  • Rats, Sprague-Dawley
  • Time Factors
  • Tongue / drug effects*
  • Tongue / physiology*

Substances

  • Nerve Tissue Proteins
  • Cocaine- and Amphetamine-Regulated Transcript Protein