Use of site-specific protein-DNA photocrosslinking to analyze the molecular organization of the RNA polymerase II initiation complex

Methods Mol Biol. 2001:148:383-93. doi: 10.1385/1-59259-208-2:383.
No abstract available

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Autoradiography
  • Base Sequence
  • Binding Sites
  • Cross-Linking Reagents
  • DNA / chemistry
  • DNA / genetics
  • DNA / metabolism*
  • DNA Primers
  • Deoxyribonucleotides / metabolism
  • Electrophoresis, Polyacrylamide Gel / methods
  • Indicators and Reagents
  • Phosphorus Radioisotopes
  • Plasmids
  • Promoter Regions, Genetic
  • RNA Polymerase II / metabolism*
  • Restriction Mapping
  • Templates, Genetic
  • Transcription Factors / chemistry
  • Transcription Factors / metabolism*
  • Transcription, Genetic* / radiation effects
  • Ultraviolet Rays

Substances

  • Cross-Linking Reagents
  • DNA Primers
  • Deoxyribonucleotides
  • Indicators and Reagents
  • Phosphorus Radioisotopes
  • Transcription Factors
  • DNA
  • RNA Polymerase II