Abstract
CD133 is a new stem cell antigen that may provide an alternative to CD34 for the selection and expansion of hematopoietic cells for transplantation. This study compared the expansion capacities of CD133(+) and CD34(+) cells isolated from the same cord blood (CB) samples. After 14 days culture in stroma-free, serum-free medium in the presence of stem cell factor (SCF), Flt3-1, megakaryocyte growth and development factor (MGDF), and granulocyte colony-stimulating factor (G-CSF), the CD133(+) and CD34(+) fractions displayed comparable expansion of the myeloid compartment (CFC, LTC-IC, and E-LTC-IC). The expansion of CD133(+) CB cells was up to 1262-fold for total cells, 99-fold for CD34(+) cells, 109-fold for CD34(+) CD133(+) cells, 133-fold for CFU-GM, 14.5-fold for LTC-IC, and 7.5-fold for E-LTC-IC. Moreover, the expanded population was able to generate lymphoid B (CD19(+)), NK (CD56(+)), and T (CD4(+) CD8(+)) cells in liquid or fetal thymic organ cultures, while expression of the homing antigen CXCR4 was similar on expanded and nonexpanded CD133(+) or CD34(+) cells. Thus, the CD133(+) subset could be expanded in the same manner as the CD34(+) subset and conserved its multilineage capacity, which would support the relevance of CD133 for clinical hematopoietic selection.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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AC133 Antigen
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Antigens, CD
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Antigens, CD34 / analysis*
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Antigens, Surface / analysis*
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B-Lymphocytes / cytology
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B-Lymphocytes / immunology
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Cell Culture Techniques / methods
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Cell Differentiation / drug effects
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Cell Differentiation / physiology*
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Cell Division
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Cells, Cultured
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Culture Media, Serum-Free
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Fetal Blood / cytology*
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Flow Cytometry
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Glycoproteins / analysis*
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Granulocyte Colony-Stimulating Factor / pharmacology
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Hematopoietic Stem Cells / cytology*
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Hematopoietic Stem Cells / drug effects
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Hematopoietic Stem Cells / immunology
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Humans
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Immunomagnetic Separation / methods
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Infant, Newborn
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Killer Cells, Natural / cytology
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Killer Cells, Natural / immunology
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Organ Culture Techniques
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Peptides / analysis*
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Proto-Oncogene Proteins / pharmacology
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Receptor Protein-Tyrosine Kinases / pharmacology
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Recombinant Proteins / pharmacology
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Stem Cell Factor / pharmacology
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T-Lymphocytes / cytology
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T-Lymphocytes / immunology
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Thrombopoietin / pharmacology
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Thymus Gland / embryology
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Thymus Gland / immunology
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fms-Like Tyrosine Kinase 3
Substances
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AC133 Antigen
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Antigens, CD
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Antigens, CD34
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Antigens, Surface
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Culture Media, Serum-Free
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Glycoproteins
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PROM1 protein, human
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Peptides
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Proto-Oncogene Proteins
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Recombinant Proteins
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Stem Cell Factor
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Granulocyte Colony-Stimulating Factor
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Thrombopoietin
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FLT3 protein, human
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Receptor Protein-Tyrosine Kinases
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fms-Like Tyrosine Kinase 3