A novel early estrogen-regulated gene gec1 encodes a protein related to GABARAP

Biochem Biophys Res Commun. 2001 Jun 1;284(1):118-25. doi: 10.1006/bbrc.2001.4908.

Abstract

We have isolated, in guinea-pig endometrial cells, an estrogen-induced 1.8 kb RNA called gec1. Screening of a guinea-pig genomic library led to identification of gec1 gene consisting of 4 exons and 3 introns. Exon 1 contains the 5'UTR and the ATG initiation codon. A guinea-pig gec1 cDNA was obtained by 5'-RACE. The 351 bp coding sequence shares 76.8% identity with that of the human GABARAP 924 bp cDNA while UTRs of the two cDNAs differ. A gec1 probe from the 3'UTR revealed a 1.9 kb mRNA in human tissues and a human GEC1 cDNA was isolated from placenta. Its coding sequence shares 93 and 79% identity with that of guinea-pig gec1 and human GABARAP, respectively. The human and guinea-pig GEC1 proteins have 100% identity. GEC1 and GABARAP proteins have 87% identity and N terminus featuring a tubulin binding motif. Thus, estrogen-regulated gec1 is a new gene which could encode a microtubule-associated protein.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions
  • Adaptor Proteins, Signal Transducing
  • Animals
  • Apoptosis Regulatory Proteins
  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • DNA, Complementary / isolation & purification
  • Estrogens* / pharmacology
  • Exons
  • Female
  • Gene Expression Regulation / drug effects
  • Gene Expression Regulation / physiology*
  • Genomic Library
  • Guinea Pigs
  • Humans
  • Introns
  • Microtubule-Associated Proteins / biosynthesis
  • Microtubule-Associated Proteins / genetics*
  • Molecular Sequence Data
  • Organ Specificity
  • Placenta
  • RNA, Messenger / biosynthesis
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid
  • Transcription, Genetic* / drug effects

Substances

  • 3' Untranslated Regions
  • Adaptor Proteins, Signal Transducing
  • Apoptosis Regulatory Proteins
  • DNA, Complementary
  • Estrogens
  • GABARAP protein, human
  • GABARAPL1 protein, human
  • Microtubule-Associated Proteins
  • RNA, Messenger

Associated data

  • GENBANK/AF012920
  • GENBANK/AF287012
  • GENBANK/AF312680