A cyclic AMP-regulated negative feedforward system for neuritogenesis revealed in a neuroblastomaxglioma hybrid cell line

Neuroscience. 2001;104(2):583-91. doi: 10.1016/s0306-4522(01)00061-6.

Abstract

We examined the role of second messengers during the neuritogenesis that accompanies neuronal differentiation in a neuroblastomaxglioma hybrid cell line (NG108-15). NG108-15 cells extended neurites after treatment with dibutyryl cyclic AMP. This dibutyryl cyclic AMP treatment evoked the synthesis of voltage-dependent Ca(2+) channel proteins in the cells. The number of neurites was decreased by Ca(2+) influx under condition of high K(+). Interestingly, the increase of neurites stimulated by dibutyryl cyclic AMP and the decrease of neurites caused by high K(+) were both reversible. This is the first study to demonstrate that cyclic AMP regulates a negative feedforward system for neuritogenesis, which links with Ca(2+) signaling. Such a dual role of cyclic AMP may play an important part in precise neurite targeting.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bucladesine / pharmacology
  • Calcium Channel Blockers / pharmacology
  • Calcium Channels / drug effects
  • Calcium Channels / metabolism*
  • Calcium Channels, L-Type / drug effects
  • Calcium Channels, L-Type / metabolism
  • Calcium Channels, N-Type / drug effects
  • Calcium Channels, N-Type / metabolism
  • Calcium Signaling / drug effects
  • Calcium Signaling / physiology*
  • Cell Differentiation / drug effects
  • Cell Differentiation / physiology*
  • Chimera / metabolism
  • Cyclic AMP / analogs & derivatives
  • Cyclic AMP / metabolism*
  • Fluorescent Dyes / pharmacokinetics
  • Fura-2 / pharmacokinetics
  • Glioma
  • Immunohistochemistry
  • Intracellular Fluid / drug effects
  • Intracellular Fluid / metabolism
  • Mice
  • Nervous System / cytology
  • Nervous System / embryology
  • Nervous System / metabolism
  • Neurites / drug effects
  • Neurites / metabolism*
  • Neurites / ultrastructure
  • Neuroblastoma
  • Potassium Chloride / pharmacokinetics
  • Rats
  • Second Messenger Systems / drug effects
  • Second Messenger Systems / physiology
  • Signal Transduction / drug effects
  • Signal Transduction / physiology
  • Tumor Cells, Cultured / cytology
  • Tumor Cells, Cultured / drug effects
  • Tumor Cells, Cultured / metabolism*

Substances

  • Calcium Channel Blockers
  • Calcium Channels
  • Calcium Channels, L-Type
  • Calcium Channels, N-Type
  • Fluorescent Dyes
  • Bucladesine
  • Potassium Chloride
  • Cyclic AMP
  • Fura-2