First outbreak of callitrichid hepatitis in Germany: genetic characterization of the causative lymphocytic choriomeningitis virus strains

Virology. 2001 Jun 5;284(2):203-13. doi: 10.1006/viro.2001.0909.

Abstract

Callitrichid hepatitis (CH) is a highly fatal, rodent-borne zoonosis of New World primates (family Callitrichidae) caused by lymphocytic choriomeningitis virus (LCMV). It is unclear whether virulence in Callitrichidae is associated with specific genetic or phylogenetic markers of the virus as only a partial S RNA sequence of a single CH-associated isolate is known. In a period of 10 months, three pygmy marmosets (Cebuella pygmaea) and one Goeldi's monkey (Callimico goeldii) died from CH in a German zoo. LCMV was most likely transmitted by wild mice. Infection was associated with characteristic histopathological lesions in liver, brain, and lymphoid tissue. Virus sequences from all callitrichids and a captured mouse were > or =99.2% identical. LCMV strains from a pygmy marmoset and the Goeldi's monkey were isolated in cell culture and the 3.4-kb S RNA was completely sequenced. Both strains differed considerably in their genetic and phylogenetic characteristics from known LCMV strains, including the previously described CH-associated strain. These data show that CH is widespread and can be caused by distantly related LCMV strains.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Animals, Zoo / virology*
  • Base Sequence
  • Callimico / virology*
  • Callitrichinae / virology*
  • Cloning, Molecular
  • Disease Outbreaks / veterinary*
  • Fluorescent Antibody Technique
  • Genome, Viral*
  • Germany
  • Hepatitis, Viral, Animal / epidemiology
  • Hepatitis, Viral, Animal / virology*
  • Humans
  • L Cells
  • Liver / pathology
  • Liver / virology
  • Lymphocytic choriomeningitis virus / genetics
  • Lymphocytic choriomeningitis virus / isolation & purification*
  • Male
  • Mice
  • Molecular Sequence Data
  • Monkey Diseases / virology*
  • Phylogeny
  • Polymerase Chain Reaction
  • RNA, Viral / analysis
  • Sequence Alignment

Substances

  • RNA, Viral

Associated data

  • GENBANK/AF325214
  • GENBANK/AF325215