IgH, TCR-gamma, and TCR-beta gene rearrangement in 80 B- and T-cell non-Hodgkin's lymphomas: study of the association between proliferation and the so-called "aberrant" patterns

Diagn Mol Pathol. 2001 Jun;10(2):69-77. doi: 10.1097/00019606-200106000-00001.

Abstract

The current study analyzes the rearrangement pattern of immunoglobulin H (IgH), T-cell receptor (TCR)-gamma, and TCR-beta genes in a group of 80 non-Hodgkin's lymphomas (NHL) of different histologic subtypes (43 B-cell and 37 T-cell types). The sensitivity and specificity provided by polymerase chain reaction amplification of these loci are evaluated. The association between the proliferation index and the presence of the so-called "aberrant" or "dual" rearrangements is also considered. Ninety-one percent of B-cell NHL showed IgH gene monoclonality, and 21% also exhibited a monoclonal pattern in one of the TCR genes. Among T-cell NHL, the sensitivity of the study was 65% for the TCR-gamma gene and 46% for the TCR-beta gene. The total sensitivity was 76%, amplifying both loci. IgH gene aberrant rearrangements were observed in 16% of T-cell neoplasms. A substantial percentage of dual rearrangements were detected in precursor and mature B- and T-cell NHL. B-cell NHL showed a tendency toward higher values of proliferation when aberrant rearrangements were present; however, this trend was not significant. Furthermore, in the case of T-cell NHL there was a significant negative association between these two variables.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Nuclear
  • Biomarkers, Tumor / analysis
  • Cell Count
  • Cell Division
  • Clone Cells
  • DNA, Neoplasm / analysis
  • Gene Rearrangement, beta-Chain T-Cell Antigen Receptor / genetics*
  • Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor / genetics*
  • Genes, Immunoglobulin / genetics*
  • Humans
  • Immunoglobulin Heavy Chains / genetics*
  • Immunohistochemistry
  • Lymphoma, B-Cell / chemistry
  • Lymphoma, B-Cell / genetics*
  • Lymphoma, B-Cell / pathology
  • Lymphoma, T-Cell / chemistry
  • Lymphoma, T-Cell / genetics*
  • Lymphoma, T-Cell / pathology
  • Nuclear Proteins / analysis
  • Polymerase Chain Reaction
  • Sensitivity and Specificity

Substances

  • Antigens, Nuclear
  • Biomarkers, Tumor
  • DNA, Neoplasm
  • Immunoglobulin Heavy Chains
  • Nuclear Proteins