Latex allergy is progressively increasing due to the widespread use of individual protective disposals, especially latex gloves among health care workers. A multidisciplinary Working Group of the Association of Health Care Workers' Preventive Medicine published, in 1995, the Guidelines for the prevention of allergic reactions both in workers and patients. The Group identified a diagnostic protocol, including the assessment of predisposition, cross-reactions, level of exposure and symptoms; etiological diagnosis needs specific, allergologic and provocative tests. Primary prevention, pre-employment education and work restrictions for sensitised workers were particularly taken into account. A short questionnaire was prepared to identify latex sensitisation in patients undergoing invasive procedures; tests are performed only in selected subjects. Preventive measures require the use of nonlatex gloves and the identification and substitution of all latex products in health care settings; such measures are necessary when emergency procedures are performed.