Cytological effects of experimental exposure to Hg on the gill epithelium of the European flat oyster Ostrea edulis: ultrastructural and quantitative changes related to bioaccumulation

Tissue Cell. 2001 Apr;33(2):178-88. doi: 10.1054/tice.2000.0169.

Abstract

The significance of ultrastructural changes in the gill epithelial cells as a parameter of detection of Hg exposure in the flat oyster, Ostrea edulis, was tested by a 34-day exposure to 5 microg l(-1) of Hg. The concentration of Hg (38.76 microg g(-1) dry weight) in gill tissue was maximal after 25 days and then decreased. The histological pattern of gill filaments in control samples did not vary throughout the experiment, except for the volume of mucocytes after 4 days of exposure, as an adaptation to experimental conditions. This volume increased significantly and then decreased according to the accumulation of Hg in the gills. After 18 days, absorptive and ciliated cells of the gill epithelium showed blebs in microvilli membranes, discocilia and swollen mitochondrial cristae. Both cell types showed distinct cellular lysis stages after 25 days of exposure. These are the target cells of Hg and other metals and the reported hypertrophy of mucocytes increase occurs in response to pollution by Hg, which could contribute to the detoxification process.

MeSH terms

  • Animals
  • Environmental Monitoring / methods
  • Epithelial Cells / drug effects
  • Epithelial Cells / ultrastructure
  • Gills / drug effects*
  • Gills / pathology
  • Mercuric Chloride / pharmacokinetics
  • Mercuric Chloride / toxicity*
  • Microscopy, Electron
  • Mitochondrial Swelling / drug effects
  • Mucous Membrane / drug effects
  • Mucous Membrane / pathology
  • Ostreidae / drug effects*
  • Water Pollutants, Chemical / pharmacokinetics
  • Water Pollutants, Chemical / toxicity*

Substances

  • Water Pollutants, Chemical
  • Mercuric Chloride