Measurement of nephrolithiasis urinary markers by capillary electrophoresis

J Chromatogr B Biomed Sci Appl. 2001 May 5;755(1-2):287-95. doi: 10.1016/s0378-4347(01)00123-2.

Abstract

A previously developed method for screening organic acidurias by capillary electrophoresis has been validated for oxalate and citrate measurement in urine. Sample pretreatment is minimum, just acidification and centrifugation. Detection is by direct UV. Validation parameters of the method can be considered adequate. Response is linear for both analytes in standards and samples. The assayed ranges were 200-1,000 mg/l for citrate and 10-200 mg/l for oxalate. Recoveries ranged from 99.4+/-3 to 101.7+/-2.4%, maximum imprecision in oxalate concentration was of 7.6% RSD and limits of detection in samples were 0.67 mg/l for oxalate and 25.9 mg/l for citrate, both lower than the measured values in samples. Identification of increased glyoxylic (oxoacetic acid) and glyceric acids (2,3-dihydroxy propanoic) are also included to facilitate the diagnosis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers / urine
  • Citric Acid / urine*
  • Electrophoresis, Capillary / methods*
  • Glyceric Acids / urine*
  • Glyoxylates / urine*
  • Humans
  • Kidney Calculi / urine*
  • Oxalates / urine*
  • Reproducibility of Results

Substances

  • Biomarkers
  • Glyceric Acids
  • Glyoxylates
  • Oxalates
  • Citric Acid
  • glyceric acid
  • glyoxylic acid