Localization of CD36 and scavenger receptor class A in human coronary arteries--a possible difference in the contribution of both receptors to plaque formation

Atherosclerosis. 2001 Jun;156(2):297-305. doi: 10.1016/s0021-9150(00)00662-6.

Abstract

CD36 and scavenger receptor class A types I and II (SR-AI/II) are major receptors for oxidized low density lipoproteins (OxLDL) expressed on macrophages. To elucidate the role of these two macrophage scavenger receptors in the development of coronary atherosclerosis, we examined the localization of CD36 and SR-AI/II in human coronary atherosclerotic lesions. Serial cryostat sections of 49 coronary arteries obtained from 43 autopsied cases were examined immunohistochemically. Regarding the relationship between the severity of atherosclerosis and immunoreactivities to CD36, there was almost no immunoreactivity to CD36 in regions with diffuse intimal thickening, while the expression of CD36 was accelerated in parallel with the progression of atherosclerosis. In contrast, SR-AI/II was expressed persistently from regions with diffuse intimal thickening to atherosclerotic plaques. We also clarified the differential distribution of CD36 and SR-AI/II in atheromatous plaques. Close to the luminal surface of the intima, macrophages were relatively small in size, contained lesser lipids, and expressed SR-AI/II more abundantly than CD36. In contrast, macrophages in the core region were larger in size, contained more lipids, were strongly positive for CD36 and showed a weaker immunoreactivity to SR-AI/II than those in the luminal surface of the intima. In conclusion, the expression of CD36 and SR-AI/II on macrophages may be regulated differently in the process of coronary atherogenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Age Factors
  • Aged
  • Aged, 80 and over
  • CD36 Antigens / analysis*
  • Child
  • Child, Preschool
  • Coronary Artery Disease / metabolism*
  • Coronary Artery Disease / pathology*
  • Coronary Vessels / immunology
  • Coronary Vessels / metabolism*
  • Coronary Vessels / pathology
  • Culture Techniques
  • Female
  • Humans
  • Immunohistochemistry
  • Infant
  • Macrophages / immunology
  • Macrophages / pathology
  • Male
  • Membrane Proteins*
  • Middle Aged
  • Receptors, Immunologic / analysis*
  • Receptors, Lipoprotein*
  • Receptors, Scavenger
  • Scavenger Receptors, Class A
  • Scavenger Receptors, Class B
  • Sensitivity and Specificity
  • Severity of Illness Index

Substances

  • CD36 Antigens
  • Membrane Proteins
  • Receptors, Immunologic
  • Receptors, Lipoprotein
  • Receptors, Scavenger
  • Scarb1 protein, mouse
  • Scavenger Receptors, Class A
  • Scavenger Receptors, Class B