Measurement of GTP-bound Ras-like GTPases by activation-specific probes

Methods Enzymol. 2001:333:343-8. doi: 10.1016/s0076-6879(01)33068-9.

Authors

M van Triest¹, J de Rooij, J L Bos

Affiliation

¹ Department of Physiological Chemistry and Centre for Biomedical Genetics, University Medical Centre Utrecht, Utrecht 3584 CG, The Netherlands.

PMID: 11400350
DOI: 10.1016/s0076-6879(01)33068-9

No abstract available

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antibodies
Blood Platelets / metabolism
Cells, Cultured
Electrophoresis, Polyacrylamide Gel
Enzyme Activation
Glutathione Transferase / genetics
Guanosine Diphosphate / metabolism*
Guanosine Triphosphate / metabolism*
Humans
Monomeric GTP-Binding Proteins / analysis
Monomeric GTP-Binding Proteins / genetics
Monomeric GTP-Binding Proteins / metabolism*
Precipitin Tests
Protein Binding
Protein Structure, Tertiary
Recombinant Fusion Proteins / analysis
Recombinant Fusion Proteins / metabolism
Substrate Specificity

Substances

Antibodies
Recombinant Fusion Proteins
Guanosine Diphosphate
Guanosine Triphosphate
Glutathione Transferase
Monomeric GTP-Binding Proteins