Abstract
The global response regulator GacA of Pseudomonas aeruginosa PAO1 positively controls the production of the quorum sensing signal molecule N-butanoyl-homoserine-lactone (C4-HSL) and hence the synthesis of several C4-HSL-dependent virulence factors, including hydrogen cyanide (HCN). This study presents evidence that GacA positively influences the transcription of the rhlI gene, specifying C4-HSL synthase, explaining the quorum sensing-dependent transcriptional control of the HCN biosynthetic genes (hcnABC). In addition, GacA was found to modulate hcn gene expression positively at a post-transcriptional level involving the hcnA ribosome-binding site. Thus, the activating effect of GacA on cyanogenesis results from both transcriptional and post-transcriptional mechanisms.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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4-Butyrolactone / analogs & derivatives
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4-Butyrolactone / biosynthesis
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4-Butyrolactone / genetics*
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism*
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Gene Expression Regulation, Bacterial
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Hydrogen Cyanide / metabolism*
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Multienzyme Complexes / biosynthesis
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Multienzyme Complexes / genetics*
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Oxidoreductases / biosynthesis
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Oxidoreductases / genetics*
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Oxidoreductases Acting on CH-NH2 Group Donors
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Pseudomonas aeruginosa / enzymology
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Pseudomonas aeruginosa / genetics*
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RNA Processing, Post-Transcriptional
Substances
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Bacterial Proteins
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GacA protein, Bacteria
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Multienzyme Complexes
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N-butyrylhomoserine lactone
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RhiI protein, Rhizobium leguminosarum
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Hydrogen Cyanide
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Oxidoreductases
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Oxidoreductases Acting on CH-NH2 Group Donors
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glycine dehydrogenase (cyanide-forming)
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4-Butyrolactone