The composting of high organic content wastes has been shown to produce nitrous oxide (N2O). This study was initiated to investigate the mechanisms of N2O emissions from aerated composting and to determine the optimal operational conditions that minimize N2O emissions. The results of our experiment in laboratory-scale composters showed that more than 95% of N2O was produced during the later period of composting when readily available carbon sources had been depleted. Significant increases in N2O emission after nitrite (NO2-) addition, and good NO(2-)-N2O correlation, indicates that N2O was transformed from NO2-. Extremely high N2O generation was observed after NO2- addition in the presence and absence of composted cattle manure. This suggests an identical mechanism for N2O production in both treatments. However, the addition of composted cattle manure resulted in an earlier initiation of the main N2O generation period. Intermittent feeding of fresh food waste postponed the main N2O generation period, and reduced the mass-based N2O emissions by 20%.