Migration and multipotentiality of PSA-NCAM+ neural precursors transplanted in the developing brain

Mol Cell Neurosci. 2001 Jun;17(6):983-1000. doi: 10.1006/mcne.2001.0987.

Abstract

By optimizing the previously described strategy for obtention of spheres enriched in PSA-NCAM+ precursors, we prepared PSA-NCAM-immunoselected cell populations from cerebral hemispheres of neonatal MBP-LacZ transgenic mice. These cells expressed Nestin, exhibited clonal expansion potential and formed spheres, which were initially enriched in PSA-NCAM+ cells but became enriched in GD3+ oligodendrocyte progenitors after 1 week in B104 contionned medium. One month after their periventricular transplantation into the brain of wild-type and/or shiverer newborn mice, cells from PSA-NCAM+ spheres exhibited a higher rostral migration potential than cells from GD3+ spheres, and clearly contributed to myelination in the olfactory bulb. In shiverer hosts, both sphere populations generated oligodendrocytes with similar myelination potential. In addition PSA-NCAM+ sphere cells generated GFAP+ astrocytes and NeuN+ neurons, depending on their site of insertion. These results evidence the high plasticity of newborn PSA-NCAM+ neural precursors and suggest that they are promising tools for cell therapy of CNS diseases, including myelin disorders.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Differentiation / metabolism
  • Astrocytes / cytology
  • Astrocytes / metabolism
  • Brain / cytology
  • Brain / growth & development*
  • Brain / metabolism
  • Brain Tissue Transplantation / methods
  • Cell Aggregation / genetics
  • Cell Culture Techniques / methods
  • Cell Differentiation / physiology*
  • Cell Lineage / physiology*
  • Cell Movement / physiology*
  • Cells, Cultured / cytology
  • Cells, Cultured / drug effects
  • Cells, Cultured / metabolism
  • Central Nervous System Diseases / surgery
  • Clone Cells / cytology
  • Clone Cells / drug effects
  • Clone Cells / metabolism
  • Culture Media / pharmacology
  • Gangliosides / metabolism
  • Gene Expression Regulation, Developmental / physiology
  • Glial Fibrillary Acidic Protein / metabolism
  • Graft Survival / physiology
  • Intermediate Filament Proteins / metabolism
  • Mice
  • Mice, Transgenic
  • Nerve Tissue Proteins*
  • Nestin
  • Neural Cell Adhesion Molecule L1*
  • Neural Cell Adhesion Molecules / genetics
  • Neural Cell Adhesion Molecules / metabolism*
  • Neurons / cytology
  • Neurons / metabolism
  • Neurons / transplantation*
  • Oligodendroglia / cytology
  • Oligodendroglia / metabolism
  • Sialic Acids / genetics
  • Sialic Acids / metabolism*
  • Stem Cell Transplantation*
  • Stem Cells / cytology
  • Stem Cells / metabolism
  • Thalamus / cytology
  • Thalamus / growth & development
  • Thalamus / surgery

Substances

  • Antigens, Differentiation
  • Culture Media
  • Gangliosides
  • Glial Fibrillary Acidic Protein
  • Intermediate Filament Proteins
  • Nerve Tissue Proteins
  • Nes protein, mouse
  • Nestin
  • Neural Cell Adhesion Molecule L1
  • Neural Cell Adhesion Molecules
  • Sialic Acids
  • oligodendrocyte O antigen, mouse
  • polysialyl neural cell adhesion molecule
  • ganglioside, GD3