Determination of the primary structure of two lipid transfer proteins from apricot (Prunus armeniaca)

J Chromatogr B Biomed Sci Appl. 2001 May 25;756(1-2):123-9. doi: 10.1016/s0378-4347(01)00097-4.

Abstract

It has been recently demonstrated that the major allergen of apricot is a protein of molecular mass (Mr) 9000 belonging to the family of Lipid Transfer Protein. The aim of this study was the determination of the primary structure of apricot LTP by micro-sequencing and mass spectrometric analyses. Apricot LTP is a 91 amino acids protein like peach and almond LTPs with a sequence identity of 91% and 94%, respectively. Like for the peach LTP, out of the 25 amino acids forming the inner surface of the tunnel-like hydrophobic cavity in maize ns-LTP, 16 are identical and 7 similar in the apricot LTP, supporting the hypothesis of a similar function.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antigens, Plant
  • Carrier Proteins / chemistry*
  • Carrier Proteins / immunology
  • Chromatography, High Pressure Liquid
  • Cross Reactions
  • Food Hypersensitivity
  • Mice
  • Molecular Sequence Data
  • Plant Proteins
  • Rosales / chemistry*
  • Rosales / immunology
  • Sequence Homology, Amino Acid
  • Spectrometry, Mass, Electrospray Ionization

Substances

  • Antigens, Plant
  • Carrier Proteins
  • Plant Proteins
  • lipid transfer proteins, plant