Abstract
The symbiotically important trimer of the succinoglycan octasaccharide subunit was labeled with a biotin tag through coupling with a 6-biotinamidohexan hydrazide and subsequent reduction with borane. The acetyl and succinyl groups in the molecule were stable to the two-step sequence, while a small percentage of the ketal in the pyruvate groups was reduced to an ether-linked lactic acid moiety attached to either the O-4 or O-6 position of the sugar residue under the reaction conditions.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Biotin
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Carbohydrate Sequence
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Chromatography, Ion Exchange
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Magnetic Resonance Spectroscopy
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Medicago sativa / microbiology
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Molecular Sequence Data
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Oligosaccharides / chemistry*
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Oxidation-Reduction
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Polysaccharides, Bacterial / chemistry*
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Signal Transduction
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Sinorhizobium meliloti / chemistry*
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Sinorhizobium meliloti / physiology
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Symbiosis
Substances
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Oligosaccharides
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Polysaccharides, Bacterial
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Biotin
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succinoglycan