Incorporation of alpha- and beta-LNA (locked nucleic acid) monomers in oligodeoxynucleotides with polarity reversals

Bioorg Med Chem Lett. 2001 Jul 9;11(13):1765-8. doi: 10.1016/s0960-894x(01)00298-0.

Abstract

The thymidine monomers of LNA with both alpha- and beta-configuration are incorporated with polarity reversals (i.e., with 3'-3' and 5'-5' junctions) in oligodeoxynucleotides with beta- and alpha-configuration, respectively. A 5'-O-phosphoramidite of the beta-LNA monomer is synthesised. Large destabilisations of duplexes with both complementary DNA and RNA are observed for oligodeoxynucleotides containing the alpha-LNA monomer, whereas a duplex with complementary RNA of an alpha-oligodeoxynucleotide containing the beta-LNA monomer is not destabilised.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Nucleic Acids / chemistry*
  • Oligodeoxyribonucleotides / chemistry*

Substances

  • Nucleic Acids
  • Oligodeoxyribonucleotides