A recombinant Semliki Forest virus particle vaccine encoding the prME and NS1 proteins of louping ill virus is effective in a sheep challenge model

M M Morris-Downes; B J Sheahan; M N Fleeton; P Liljeström; H W Reid; G J Atkins

doi:10.1016/s0264-410x(01)00126-8

A recombinant Semliki Forest virus particle vaccine encoding the prME and NS1 proteins of louping ill virus is effective in a sheep challenge model

Vaccine. 2001 Jul 16;19(28-29):3877-84. doi: 10.1016/s0264-410x(01)00126-8.

Authors

M M Morris-Downes¹, B J Sheahan, M N Fleeton, P Liljeström, H W Reid, G J Atkins

Affiliation

¹ Department of Microbiology, Moyne Institute of Preventive Medicine, Trinity College, Dublin 2, Ireland.

PMID: 11427261
DOI: 10.1016/s0264-410x(01)00126-8

Abstract

This study has examined the efficacy following intramuscular administration of a recombinant Semliki Forest virus (rSFV) vaccine, encoding the prME and NS1 proteins of louping ill virus (LIV), in sheep. Administration of rSFV-LIV vaccine resulted in transient detection at the injection site and draining lymph node only and no dissemination to distal sites. In addition, the recombinant vaccine offered complete protection against subcutaneous challenge with LIV, and partial protection following intranasal administration of LIV. Protected animals had no pathological changes normally associated with LIV infection, and had developed high antibody titres. In contrast, the two animals not protected exhibited classical clinical signs and neuropathological lesions of LIV infection. These findings indicate that rSFV-based vaccines have the potential to be developed as effective prototype vaccines for LIV.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies, Viral / blood
Base Sequence
DNA Primers / genetics
Encephalitis Viruses, Tick-Borne / genetics*
Encephalitis Viruses, Tick-Borne / immunology*
Encephalitis, Tick-Borne / immunology
Encephalitis, Tick-Borne / pathology
Encephalitis, Tick-Borne / prevention & control
Encephalitis, Tick-Borne / veterinary
Genetic Vectors
Reverse Transcriptase Polymerase Chain Reaction
Semliki forest virus / genetics*
Semliki forest virus / immunology*
Sheep
Sheep Diseases / immunology
Sheep Diseases / pathology
Sheep Diseases / prevention & control
Vaccines, Synthetic / genetics
Vaccines, Synthetic / immunology
Vaccines, Synthetic / pharmacology
Viral Nonstructural Proteins / genetics*
Viral Nonstructural Proteins / immunology*
Viral Structural Proteins / genetics*
Viral Structural Proteins / immunology*
Viral Vaccines / genetics
Viral Vaccines / immunology
Viral Vaccines / pharmacology

Substances

Antibodies, Viral
DNA Primers
Vaccines, Synthetic
Viral Nonstructural Proteins
Viral Structural Proteins
Viral Vaccines