Improved detection of methicillin-resistant Staphylococcus aureus using phenyl mannitol broth containing aztreonam and ceftizoxime

H Wertheim; H A Verbrugh; C van Pelt; P de Man; A van Belkum; M C Vos

doi:10.1128/JCM.39.7.2660-2662.2001

Improved detection of methicillin-resistant Staphylococcus aureus using phenyl mannitol broth containing aztreonam and ceftizoxime

J Clin Microbiol. 2001 Jul;39(7):2660-2. doi: 10.1128/JCM.39.7.2660-2662.2001.

Authors

H Wertheim¹, H A Verbrugh, C van Pelt, P de Man, A van Belkum, M C Vos

Affiliation

¹ Infectious Diseases, Erasmus University Medical Center Rotterdam, Rotterdam, The Netherlands. [email protected]

Abstract

We tested a phenyl mannitol broth containing ceftizoxime and aztreonam (PHMB(+)) for detection of methicillin-resistant Staphylococcus aureus (MRSA) with reference MRSA strains and, subsequently, with clinical samples (n = 1,098). All reference MRSA strains induced color change in PHMB(+) after 24 to 72 h of incubation. In a clinical setting, 40 MRSA strains were detected with PHMB(+), compared with only 23 detected with a routine method. Thus, this selective broth significantly (P < 0.001) improved the rate of MRSA detection.

Publication types

Evaluation Study

MeSH terms

Aztreonam / pharmacology
Bacteriological Techniques
Ceftizoxime / pharmacology
Cephalosporins / pharmacology
Culture Media / chemistry
Humans
Mannitol / metabolism*
Methicillin Resistance*
Monobactams / pharmacology
Phenolsulfonphthalein / metabolism*
Staphylococcal Infections / microbiology
Staphylococcus aureus / classification
Staphylococcus aureus / drug effects*
Staphylococcus aureus / growth & development
Staphylococcus aureus / isolation & purification*

Substances

Cephalosporins
Culture Media
Monobactams
Mannitol
Ceftizoxime
Aztreonam
Phenolsulfonphthalein