Abstract
Protease-activated receptor 1 (PAR-1) is a G-coupled membrane protein. In this study, we analyzed the expression of PAR-1 in oral squamous cell carcinomas (SCCs). PAR-1 was expressed in oral SCCs, but the level of PAR-1 protein was lower in non-metastatic cells than in metastatic cells. Thrombin stimulated the growth of metastatic cells, and both thrombin and thrombin receptor activation peptide (TRP) enhanced the adhesion of these cells to fibronectin, but had no effect on non-metastatic cells. Thrombin and TRP also induced matrix metalloproteinase (MMP)-2 and MMP-9 activities in metastatic cells. These results suggest that PAR-1 may contribute to the growth and invasive potential of oral SCC.
MeSH terms
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Blotting, Western
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Caenorhabditis elegans Proteins*
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Carcinoma, Squamous Cell / metabolism*
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Cell Adhesion
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Cell Division
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Cell Line
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Fibronectins / metabolism
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Humans
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Immunohistochemistry
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Matrix Metalloproteinase 2 / biosynthesis
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Matrix Metalloproteinase 9 / biosynthesis
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Matrix Metalloproteinases / metabolism
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Mouth Neoplasms / metabolism*
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Protein Serine-Threonine Kinases / metabolism
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Proteins / pharmacology
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RNA, Messenger / metabolism
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Receptor, PAR-1
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Receptors, Thrombin / biosynthesis*
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Reverse Transcriptase Polymerase Chain Reaction
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Thrombin / metabolism
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Thrombin / pharmacology
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Tumor Cells, Cultured
Substances
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Caenorhabditis elegans Proteins
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Fibronectins
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Proteins
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RNA, Messenger
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Receptor, PAR-1
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Receptors, Thrombin
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Protein Serine-Threonine Kinases
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Thrombin
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Matrix Metalloproteinases
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Matrix Metalloproteinase 2
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Matrix Metalloproteinase 9