Abstract
A role for Stat4 in IL-12-induced up-regulation of selectin ligands on Th1 cells was explored. Th1 cells generated from Stat4(-/-) mice exhibited no IL-12-inducible P-selectin ligands, no up-regulation of core 2 beta1,6-glucosaminyltransferase I (C2GlcNAcT-I), and low levels of the Th1 transcription factor T-bet. In contrast, Stat4(-/-) Th1 cells exhibited only a partial defect in expression of IL-12-inducible E-selectin ligands and expressed equivalently high levels of alpha1,3-fucosyltransferase VII (FucT-VII) as wild-type Th1 cells. FucT-VII expression was induced by T cell activation, and was enhanced by IL-12 independently of Stat4, whereas C2GlcNAcT-I up-regulation was mediated exclusively by IL-12, acting through Stat4. These data show that FucT-VII and C2GlcNAcT-I are controlled through distinct pathways and imply the existence of at least one other IL-12-inducible glycosyltransferase required for E-selectin and possibly P-selectin ligand formation in Th1 cells.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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DNA-Binding Proteins / deficiency
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / physiology*
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Fucosyltransferases / biosynthesis
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Fucosyltransferases / physiology
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Ligands
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Mice
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Mice, Inbred BALB C
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Mice, Knockout
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N-Acetylglucosaminyltransferases / biosynthesis*
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N-Acetylglucosaminyltransferases / physiology
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STAT4 Transcription Factor
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Selectins / biosynthesis
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Selectins / metabolism*
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Signal Transduction / genetics
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Signal Transduction / immunology
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Th1 Cells / enzymology*
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Th1 Cells / metabolism
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Trans-Activators / deficiency
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Trans-Activators / genetics
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Trans-Activators / physiology*
Substances
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DNA-Binding Proteins
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Ligands
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STAT4 Transcription Factor
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Selectins
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Stat4 protein, mouse
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Trans-Activators
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Fucosyltransferases
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N-Acetylglucosaminyltransferases
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beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-acetylglucosaminyl transferase
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galactoside 3-fucosyltransferase