Abstract
We studied the regulation of the expression of vascular endothelial growth factor (VEGF) by TSH in monolayer cultures of rat thyroid FRTL-5 cell lines. The VEGF mRNA synthesis was significantly inhibited by TSH as well as dibutyryl cyclic adenosine monophosphate (cAMP) in a dose-dependent manner in FRTL-5 cells. This observation is contrary to previously published results using the thyroid follicular culture system. Our results suggest that the direct effect of TSH/cAMP is to inhibit the VEGF synthesis in monolayer cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Bucladesine / pharmacology
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Cell Line
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Down-Regulation
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Endothelial Growth Factors / genetics
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Endothelial Growth Factors / metabolism*
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Lymphokines / genetics
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Lymphokines / metabolism*
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RNA, Messenger / metabolism
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Rats
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Thyrotropin / pharmacology*
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Vascular Endothelial Growth Factor A
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Vascular Endothelial Growth Factors
Substances
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Endothelial Growth Factors
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Lymphokines
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RNA, Messenger
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Vascular Endothelial Growth Factor A
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Vascular Endothelial Growth Factors
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Bucladesine
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Thyrotropin