Cytokine mRNA expression in mouse colon: IL-15 mRNA is overexpressed and is highly sensitive to a fibre-like dietary component (short-chain fructo-oligosaccharides) in an Apc gene manner

Cytokine. 2001 May 21;14(4):243-6. doi: 10.1006/cyto.2001.0872.

Abstract

On the basis of studies using the Min mouse model of colon carcinogenesis, we have recently proposed that a fibre-like food (short-chain fructo-oligosaccharides, sc-FOS) fermented in the colon may stimulate a mechanism of cancer immunosurveillance. In the present paper, we have investigated the expression of cytokines as potential effector molecules. Interleukin (IL-)4, IL-5, IL-13, IL-15 and interferon (INF)-gamma mRNAs were detected by a multi-probe ribonuclease protection assay in C57BL/6J and Min mouse colons. IL-15 mRNA expression was significantly amplified (P=0.01) by the sc-FOS-enriched diet in the colon of Min mice.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Colon / immunology*
  • Colon / metabolism*
  • Cytokines / biosynthesis
  • Diet
  • Dietary Fiber / administration & dosage*
  • Genes, APC*
  • Interleukin-15 / biosynthesis*
  • Interleukin-15 / genetics
  • Mice
  • Mice, Inbred C57BL
  • Mice, Mutant Strains
  • Oligosaccharides / administration & dosage*
  • RNA, Messenger / biosynthesis*

Substances

  • Cytokines
  • Dietary Fiber
  • Interleukin-15
  • Oligosaccharides
  • RNA, Messenger
  • fructooligosaccharide