Synthesis of linear and comb-like peptide constructs containing up to four copies of a T cell epitope and their capacity to stimulate T cells

J Mack; K Falk; O Rötzschke; T Walk; J L Strominger; G Jung

doi:10.1002/psc.327

Synthesis of linear and comb-like peptide constructs containing up to four copies of a T cell epitope and their capacity to stimulate T cells

J Pept Sci. 2001 Jun;7(6):338-45. doi: 10.1002/psc.327.

Authors

J Mack¹, K Falk, O Rötzschke, T Walk, J L Strominger, G Jung

Affiliation

¹ Institute of Organic Chemistry, University of Tübingen, Germany.

PMID: 11461048
DOI: 10.1002/psc.327

Abstract

Polypeptide constructs containing up to four copies of the T cell epitope 306-318 of influenza virus haemagglutinin have been synthesized on solid phase. Between the copies, a non-natural PEG-based spacer amino acid has been introduced. The oligomeric epitopes were analysed by RP-HPLC and ES-MS. The arrangement of the epitopes within the peptide constructs was either linear or comb-like. The proliferative response in a T helper cell assay induced by these oligomerized epitopes has been tested, showing that the linearly arranged epitopes are more effective than the comb-like oligomers.

MeSH terms

Amino Acid Sequence
Antigens, Viral / chemistry
Antigens, Viral / immunology
CD4-Positive T-Lymphocytes / cytology
CD4-Positive T-Lymphocytes / immunology*
Cell Division
Cell Line
Chromatography, High Pressure Liquid
Epitopes, T-Lymphocyte / chemistry*
Epitopes, T-Lymphocyte / immunology*
Hemagglutinin Glycoproteins, Influenza Virus / chemistry
Hemagglutinin Glycoproteins, Influenza Virus / immunology*
Humans
Leukocytes, Mononuclear / cytology
Leukocytes, Mononuclear / immunology
Mass Spectrometry
Peptides / chemical synthesis*
Peptides / chemistry
Peptides / immunology*
Protein Conformation
Repetitive Sequences, Amino Acid / immunology*
Structure-Activity Relationship

Substances

Antigens, Viral
Epitopes, T-Lymphocyte
Hemagglutinin Glycoproteins, Influenza Virus
Peptides